02280nam a22002177a 4500 D0001C000056 DOH 20201121114613.0 201121b ||||| |||| 00| 0 eng d Severe acute respiratory syndrome coronavirus spike protein counteracts BST2‐mediated restriction of virus‐like particle release / Shiu‐Mei Wang [and two others] BST2/tetherin, an interferon-inducible antiviral factor, can block the cellular release of various enveloped viruses. We previously reported that human coronavirus 229E (HCoV-229E) infection can alleviate the BST2 tethering of HIV-1 virions by downregulating cell surface BST2, suggesting that coronaviruses are capable of encoding anti-BST2 factors. Here we report our new finding that severe acute respiratory syndrome coronavirus (SARS-CoV) spike (S) glycoprotein, similar to Vpu, is capable of antagonizing the BST2 tethering of SARS-CoV, HCoV-229E, and HIV-1 virus-like particles via BST2 downregulation. However, unlike Vpu (which downmodulates BST2 by means of proteasomal and lysosomal degradation pathways), BST2 downregulation is apparently mediated by SARS-CoV S through the lysosomal degradation pathway only. We found that SARS-CoV S colocalized with both BST2 and reduced cell surface BST2, suggesting an association between SARS-CoV S and BST2 that targets the lysosomal degradation pathway. According to one recent report, SARS-CoV ORF7a antagonizes BST2 by interfering with BST2 glycosylation1 . Our data provide support for the proposal that SARS-CoV and other enveloped viruses are capable of evolving supplementary anti-BST2 factors in a manner that requires virus replication. Further experiments are required to determine whether the BST2-mediated restriction of authentic SARS-CoV virions is alleviated by the SARS-CoV spike protein. In: J Med Virol. 2019 vol. 91 (10) page: 1743–1750. Published online 2019 Jul 10. doi: 10.1002/jmv.25518 SARS Virus classification SARS Virus genetics SARS Virus metabolism Wang, Shiu‐Mei Huang, Kuo‐Jung Wangcorres, Chin‐Tien https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7166632/ CV 1789 1789 0 0 0 0 DOHCL DOHCL ElectronicRes 2020-11-21 0 COVID-19-000056 D0001C000056 2020-11-21 00:00:00 2020-11-21 CV