03681nam a22004457a 4500001001300000003000400013005001700017008004100034245014000075520219600215580005302411650001302464650001502477700002502492700003202517700002802549700002402577700002602601700002602627700002302653700002602676700002402702700002602726700002602752700002902778700002902807700002902836700002702865700002302892700003402915700002302949700002802972700002003000700001903020700002703039700002303066942000703089999001503096952012403111D0001J000145DOH20210312110613.0210312b           ||||| |||| 00| 0 eng d00aScreening for the activity of centella asiatica (L.) urb. on mice with induced depression /cPreciosa M. Coloma [and twenty two others]3 aCentella asiatica (L.) Urb was screened for its activity in mice with induced depression using the Forced Swimming Test ( FST ) and the Shuttle Box Escape Test (SBET) as models of depression. Acute toxicity test using oral crude extract preparations on mice' snowed no lethality at all doses and aminimum effective dose of 6g/kgBW, based on characterization of toxidrome. For the forced swimming test, 36 mice were preconditioned by subjecting to forced swimming for 15 minutes. Twenty- four hours later, their cumulative immobility time per 5 minutes of forced swimming was taken as baseline testing, a negative group was gavaged with flouxetine ( Prozac) 10mg/kgBW, and an experimental group was gavaged with centella asiatica crude extract 6g/kgBW. One hour after gavage, the cumulative immobility time per 5 minutes forced swimming of all three groups was measured. For the Shuttle box escape test, 30 out of 43 mice were preconditioned being subjected to 60 separate electric shocks ( 15 sec, 28V per shock); the remaining 13 mice served as negative control. Forty -eight hours later, escaped after all 43 mice was taken as baseline 23 hours after baseline testing , 15 mice served as positive control and were gavaged with Fluoxetine ( prozac) 2mg/kgBW and 15 mice served as the experimental group and were gavaged with centella extract. The remaining 14 mice served as negative control. One hour fater gavage, mean escape latencies of all 43 mice were measured. For the FST, analysis of variance of mean immobility time decrease ( MITD ) of NSS-treated mice is significantly difference between the MITD of centella-treated and prosac treated mice. For the SBET, wilcoxon tests showed no significant difference in the mean escape latencies ( MEL ) of NSS-treated and centella-treated mice. T-test showed that the MEL in baseline is not significantly higher than that of centella asiatica stimulates the CNS at doses less than 100g/kgBW and depresses the CNS at higher doses. Side effects of centella include diuresis, hypersalivation and diarrhea ( not dose related ). Using the FST and SBET, centella asistica extract used at a dose of 6g/kgBW has no anti-depressant activity in mice.  aActa Medica Philippina, 1997 33 ( 2 ) pages 1-25 2aCentella 2aDepression1 aColoma, Preciosa M. 1 aTan,  Charissa Charlene L. 1 aAlfonso, Victor J.  Jr.1 aBuenafe, Xander H. 1 aColoma, Carl Adam C. 1 aLapid Jr, Ronald  A. 1 aLeal, Dabe Roy B. 1 aManloctao, Rainer L. 1 aMiranda, Cyndee C. 1 aOliveros, Milette B. 1 aOribello, Adrian  M. 1 aResubal, Jose Rafael E. 1 aRodriguez, Cristina  P. 1 aRomano, Rico Vincent M. 1 aSabtirul, Merhamar L. 1 aSales, Shelley S. 1 aSalvador Jr III, Marcial  Q. 1 aSantos, Jhoana M. 1 aTalingdan, Marilyn  C. 1 aTana, Larry Q. 1 aUy, Norwin T. 1 aVillanueva, Ma Pia B. 1 aYango, Charina D.   cJA  c3459d3459  00102nlm4070aDOHCLbDOHCLcElectronicResd2021-03-12l0oJ000145pD0001J000145r2021-03-12 00:00:00w2021-03-12yJA