02627nam a22002777a 4500001001300000003000400013005001700017008004100034245017200075520176900247580005602016650001602072650001002088650001402098700002002112700001802132700002302150700002802173700001902201700001702220700002202237700001902259700002702278700002402305700002002329D0001J000272DOH20210322143911.0210322b           ||||| |||| 00| 0 eng d04aThe larvicidal effects of neem tree (Azadirachta indica) leaf and seed extracts on mosquito larvae (Second year school year 1993-1994) /cHenry Tiu II [and ten others]3 aJoining the global effort of making our environment a better place to live in, the researchers of this study aim to determine the larvicidal effect of the Neem tree (Azadirachta indica) leaf and seed extracts on mosquito larvae. A comparison between the seed and leaf extract as to their effectivity is also done. The research was done in the hope of achieving cheap, effective and environment-friendly insecticide.


In this study of the larvicidal effects of Neem tree leaf and seed extracts with different dilutions of 1:1, 1:2 and 1:5 are used on mosquito larvae exposed to the said extracts for different periods of time: 15 minutes, 30 minutes, 60 minutes and 120 minutes. Fresh Neem tree seeds and air-dried leaves are used as sources of the extracts. Method of extraction is by Dry-hand method. The mosquito larvae randomly collected and not taking into account their age, specie and breeding place.


T-test for the significance of two proportions is the statistical method used for the analysis of the data. The base line dilution of 1:5 was used. After three replicates with a maximum period of two hours, results showed an increasing larvicidal effect as the concentration of the Neem extract from both seed and leaf is increased. For the positive control, a 100% larvicidal effect was observed 15 minutes after the time of application. For the negative control, a larvicidal effect of zero percent was obtained even 120 minutes after the time of application. Conclusions made base on the results of the study were: that extracts from Need seeds have greater larvicidal effects on mosquito larvae than extracts from Neem leaves; that 1:1 concentrations of both Neem tree leaf and seed extract produced the greatest larvicidal effect on mosquito larvae.  aCebu Doctors' Proceedings, 1993 9 (2) pages  88-100 2aAzadirachta 2aLarva 2aCulicidae1 aTiu, Henryc II1 aWong, Elaine 1 aSon, Felipe  cJr.1 aVillamor, Cyrus Blaine 1 aPeque, Janice 1 aUy, Stanley 1 aYase, Mary Ester 1 aPadilla, Paul 1 aTremedal, Panfilo cIV1 aWong, Sydney James 1 aLumapas, Grace