01782nam a22002057a 4500 D0001J000334 DOH 20210325164301.0 210325b ||||| |||| 00| 0 eng d Determination of rifampicin in human plasma by reversed-phase liquid chromatography / Maria Lourdes C. Santiago [and three others] A simple, specific and sensitive high pressure liquid chromatographic method for the determination of rifampicin in human plasma of Filipino male and female healthy volunteers was developed and validated This method utilizes a direct precipitation separation technique and ultraviolet detection monitored at 337 nm. Dipyridamole is used as the internal standard. Rifampicin and the internal standard were separated using a Bakerbond narrow pore octyl (C8, 5um, 120 A, 4.6 x 250 nm) reversed-phase column with 10 mM phosphate buffer (pH 5.2), acetonitrile, and tetrahydrofuran (65:35:10) as the mobile phase. The retention time was 7.7 minutes for rifampicin and 14.5 minutes for dipyridamole. Results showed a linear response in the concentraion range of 0.5 to 20 mg/L (r=0.99925). The limit of quantitation is 0.5 mg/L. Both intraday and interday accuracy and precision data showed good reproducibility. The precision, accuracy, linearity, specificity, and sensitivity of the method were well-within the range of clinical utility and meet the requirements for bioavailability, bioequivalence, and pharmacokinetic studies.(Author) BFAD Laboratory Information Bulletin, 1997 3 (1) pages 30-35 Rifampin Plasma Santiago, Maria Lourdes C. Gonzales, Therese Irynne R. Sarau, Josephine K. Abejo, Vivianne O. JA 3785 3785 0 0 nlm 0 0 DOHCL DOHCL ElectronicRes 2021-03-25 0 J000334 D0001J000334 2021-03-25 00:00:00 2021-03-25 JA