01916nam a22002177a 4500001001300000003000400013005001700017008004100034245013600075520113600211580006701347650001301414650001101427700003201438700003301470700002501503700002401528942000701552999001501559952012401574D0001J000334DOH20210325164301.0210325b           ||||| |||| 00| 0 eng d00aDetermination of rifampicin in human plasma by reversed-phase liquid chromatography /cMaria Lourdes C. Santiago [and three others]3 aA simple, specific and sensitive high pressure liquid chromatographic method for the determination of rifampicin in human plasma of Filipino male and female healthy volunteers was developed and validated
This method utilizes a direct precipitation separation technique and ultraviolet detection monitored at 337 nm. Dipyridamole is used as the internal standard. Rifampicin and the internal standard were separated using a Bakerbond narrow pore octyl (C8, 5um, 120 A, 4.6 x 250 nm) reversed-phase column with 10 mM phosphate buffer (pH 5.2), acetonitrile, and tetrahydrofuran (65:35:10) as the mobile phase. The retention time was 7.7 minutes for rifampicin and 14.5 minutes for dipyridamole. Results showed a linear response in the concentraion range of 0.5 to 20 mg/L (r=0.99925). The limit of quantitation is 0.5 mg/L. Both intraday and interday accuracy and precision data showed good reproducibility.
The precision, accuracy, linearity, specificity, and sensitivity of the method were well-within the range of clinical utility and meet the requirements for bioavailability, bioequivalence, and pharmacokinetic studies.(Author)  aBFAD  Laboratory Information Bulletin, 1997  3 (1) pages 30-35 2aRifampin 2aPlasma1 aSantiago, Maria Lourdes C. 1 aGonzales, Therese Irynne R. 1 aSarau, Josephine K. 1 aAbejo, Vivianne O.   cJA  c3785d3785  00102nlm4070aDOHCLbDOHCLcElectronicResd2021-03-25l0oJ000334pD0001J000334r2021-03-25 00:00:00w2021-03-25yJA