000			02026nam a22002057a 4500
001			D0001J000003
003			DOH
005			20210206133601.0
008			210206b ||||| |||| 00| 0 eng d
245	0	0	_aMolecular characterization of hepatitis G virus (HGV) isolates from healthy adults and risk groups in the Philippines / _bJa Ricaforte-Campos [and three others]
520	3		_aHepatitis G virus (HGV) infection has been detected in Filipinos through a prospective study of 1,088 blood samples. HGV RNA was found in sera of 6/516 (1.2 percent) healthy adults (volunteer blood donors), 11/138 (8.0 percent) chronic liver disease patients, 7/207 (3.4 percent) hemodialysis patients, and 14/227 (6.2 percent) multiply transfused patients using reversetranscription polymerase chain reaction (RT-PCR) with random hexamer primers and a set of PCR primers from the 5 untranslated region (5UTR) of the HGV genome. A total of 38/1,088 subjects were HGV RNA-positive The PCR products derived from the 5UTR of HGV RNA+ samples were sequenced to determine the genotypic variant of HGV in the Philippines. Pairwise alignment of sequences and phylogenetic tree construction revealed that among the five known HGV genotypic variants, the Philippine isolates are most closely related to the Asian type (III) Considering that HGV is a highly mutable organism, surveillance for new genotypes may be the only way to assess accurate asymptomatic infection rates. How much this virus evolves in the future may have an impact on its virulence, transmissibility and invasiveness. It is therefore important, from an evolutionary perspective, to continue and monitor evolution of HGV specifically with studies at the molecular level. (Author)
580			_aIn: Acta Medica Philippina, 2005 vol. 39 (1) pages: 13-21
650		2	_aGB virus C
653			_aHepatitis G
700	1		_aRicaforte-Campos, Jane D.
700	1		_aDomingo, Ernesto O.
700	1		_aLibranda-Ramirez, Bernadette DL
700	1		_a Dalmacio, Leslie Mic,
942			_cJA
999			_c2744 _d2744