000			01782nam a22002057a 4500
001			D0001J000334
003			DOH
005			20210325164301.0
008			210325b ||||| |||| 00| 0 eng d
245	0	0	_aDetermination of rifampicin in human plasma by reversed-phase liquid chromatography / _cMaria Lourdes C. Santiago [and three others]
520	3		_aA simple, specific and sensitive high pressure liquid chromatographic method for the determination of rifampicin in human plasma of Filipino male and female healthy volunteers was developed and validated This method utilizes a direct precipitation separation technique and ultraviolet detection monitored at 337 nm. Dipyridamole is used as the internal standard. Rifampicin and the internal standard were separated using a Bakerbond narrow pore octyl (C8, 5um, 120 A, 4.6 x 250 nm) reversed-phase column with 10 mM phosphate buffer (pH 5.2), acetonitrile, and tetrahydrofuran (65:35:10) as the mobile phase. The retention time was 7.7 minutes for rifampicin and 14.5 minutes for dipyridamole. Results showed a linear response in the concentraion range of 0.5 to 20 mg/L (r=0.99925). The limit of quantitation is 0.5 mg/L. Both intraday and interday accuracy and precision data showed good reproducibility. The precision, accuracy, linearity, specificity, and sensitivity of the method were well-within the range of clinical utility and meet the requirements for bioavailability, bioequivalence, and pharmacokinetic studies.(Author)
580			_aBFAD Laboratory Information Bulletin, 1997 3 (1) pages 30-35
650		2	_aRifampin
650		2	_aPlasma
700	1		_aSantiago, Maria Lourdes C.
700	1		_aGonzales, Therese Irynne R.
700	1		_aSarau, Josephine K.
700	1		_aAbejo, Vivianne O.
942			_cJA
999			_c3785 _d3785